Journal: Life Science Alliance
Article Title: Concordance of MERFISH spatial transcriptomics with bulk and single-cell RNA sequencing
doi: 10.26508/lsa.202201701
Figure Lengend Snippet: (A) UMAP plot of MERFISH liver data (gray) with Tabula Muris Senis (teal) using scVI. Surprisingly, the two technologies are not particularly well mixed, suggesting that the underlying statistics of the two modalities are systematically different enough that constructing a good joint embedding may be difficult. (B) UMAP plot of liver integration in (A) with cell type annotations predicted using scANVI. (C) UMAP plot of MERFISH kidney data (gray) with Tabula Muris Senis (teal) using scVI. Just like the liver data, the kidney data fails to exhibit good mixing of the two modalities. (D) UMAP plot of kidney integration in (C) with cell type annotations predicted using scANVI. Cell type abbreviations in (B) are as follows: “IC,” “immune cell”; “o-EC,” “other endothelial cell”; “KC,” “Kupffer cell”; “HSC,” “hepatic stellate cell”; “o-hep,” “other hepatocyte”; “PP-hep,” “periportal hepatocyte”; “PC-hep,” “pericentral hepatocyte”; “PP-EC,” “periportal endothelial cell”; “PC-EC,” “pericentral endothelial cell.” Cell type abbreviations in (D) are as follows: “EC-PT,” “epithelial cell of proximal tubule”; “IC,” “immune cell”; “per,” “pericyte”; “KLH-EC,” “kidney loop-of-Henle epithelial cell”; “KCD-EC,” “kidney collecting-duct epithelial cell”; “KDCT-EC,” “kidney distal-convoluted-tubule epithelial cell”; “EC,” “endothelial cell,” “pod,” “podocyte.”
Article Snippet: Using segmented data, we constructed single-cell RNA count matrices to compare the single-cell MERFISH results with the 3-mo Tabula Muris Senis scRNA-seq data ( ).
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